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Somatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear ( Pyrus communis var. pyraster L.) and Colt cherry ( Prunus avium x pseudocerasus )

Identifieur interne : 003E63 ( Main/Exploration ); précédent : 003E62; suivant : 003E64

Somatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear ( Pyrus communis var. pyraster L.) and Colt cherry ( Prunus avium x pseudocerasus )

Auteurs : J. Ochatt [Royaume-Uni] ; M. Patat-Ochatt [Royaume-Uni] ; L. Rech [Royaume-Uni] ; R. Davey [Royaume-Uni] ; B. Power [Royaume-Uni]

Source :

Theoretical and Applied Genetics [ 0040-5752 ] ; 1989-07-01.

RBID : ISTEX:3837FA097FDFCB1A379BE52F7E0DE22CC642BA4B

Abstract

Summary: Mesophyll protoplasts of wild pear (Pyrus communis var. pyraster L., Pomoideae) were chemically fused with cell suspension protoplasts of cherry rootstock Colt (Prunus avium x pseudocerasus, Prunoideae), following an electroporation treatment of the separate parental protoplast systems. Fusion-treated protoplasts were cultured, on modified K8P medium, where it had been previously established that neither parental protoplasts were capable of division. Somatic hybrid calli were recovered and, following caulogenesis on MS medium with zeatin and after rooting of regenerated shoots, complete trees were obtained and grown in vivo. Hybridity of these trees was confirmed based on morphological characters, chromosome complement and isozyme analysis. Two separate cloned lines of this intersubfamilial rootstock somatic hybrid (wild pear (+) Colt cherry) were produced. This is the first report of the production of somatic hybrid plants of two woody species, of agronomic value, within the order Rosales.

Url:

https://api.istex.fr/document/3837FA097FDFCB1A379BE52F7E0DE22CC642BA4B/fulltext/pdf

DOI: 10.1007/BF00299750

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Summary: Mesophyll protoplasts of wild pear (Pyrus communis var. pyraster L., Pomoideae) were chemically fused with cell suspension protoplasts of cherry rootstock Colt (Prunus avium x pseudocerasus, Prunoideae), following an electroporation treatment of the separate parental protoplast systems. Fusion-treated protoplasts were cultured, on modified K8P medium, where it had been previously established that neither parental protoplasts were capable of division. Somatic hybrid calli were recovered and, following caulogenesis on MS medium with zeatin and after rooting of regenerated shoots, complete trees were obtained and grown in vivo. Hybridity of these trees was confirmed based on morphological characters, chromosome complement and isozyme analysis. Two separate cloned lines of this intersubfamilial rootstock somatic hybrid (wild pear (+) Colt cherry) were produced. This is the first report of the production of somatic hybrid plants of two woody species, of agronomic value, within the order Rosales.</div>
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Somatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear ( Pyrus communis var. pyraster L.) and Colt cherry ( Prunus avium x pseudocerasus )

Somatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear ( Pyrus communis var. pyraster L.) and Colt cherry ( Prunus avium x pseudocerasus )

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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